Flow Cytometry, analyzer:
Cyto Analyze,Cell Analyzer,Flow Cytometry Analyzer,Dna Cell Analyzer,Analyzer For Cell,Flow Cytometer
Flow cytometry uses flow focusing technology to focus cells in the fluid center and be irradiated by laser. Cell size, intracellular particle content and extracellular protein are analyzed by detecting the scattering light of cells and the fluorescence intensity generated by specific binding dyes.
Flow cytometry is an important tool in cell biology research.It can analyze more than 10,000 cells per second, and can conduct rapid and large-throughput statistical analysis on the size, DNA content and extracellular proteins of cells with high analysis sensitivity and more than 10 parameters analyzed at the same time. It is known as the "CT" of biological laboratory.
Cyto Analyze,Cell Analyzer,Flow Cytometry Analyzer,Dna Cell Analyzer,Analyzer For Cell,Flow Cytometer
Main Parameter of cytoanalyze, cell analyzer, Flow Cytometry, analyzer
1 Laser 488nm Solid
2 Detector FSC、SSC、FL1-FL5
3 Fluorescence channel FL1:525/(FITC) FL2:575/35(PE) FL3:615/26(PE-Texas Red/ECD/PI) FL4:692/47(PerCP/PE-Cy5/PE-Cy5.5) FL5:785/71(PE-Cy7)
4 *Detector Type APD
5 *Intake Method Peristaltic Pump
6 Fluorescence sensitivity FITC≤150MESF;PE≤50MESF
7 Fluorescence linearity ≥0.98
8 FSC sensitivity ≤1μm
9 Detected Particle Size 0.5-50μm
10 Carry Over ≤1%
11 Instrument CV FSC, all FL channel CV less than 3%
12 FSC and SSC Resolution
a）can separate red blood cell and platelet b) can separate 3 kinds of white blood cells
13 Ploid Analysis Linearity When doing diploid analysis，G2/M and G0/G1 average fluorescence intensity should be 1.95-2.05
14 CD Accuracy When run QC samples, CD3、CD4、CD8、CD16/CD56、CD19 should be within the upper/lower limit
15 CD repeatability Repeatability test on CD3、CD4、CD8、CD16/CD56、CD19 should follow：
a）when CDx≥30%，CV should be less than 8%
b）when CDx＜30%，CV should be less than 15%
16 sampling Method
(1)、Manual（Standard）
(2)、Automatic Single（Optional）
(3)、3 dimensional automatic（96 position）（Optional）
(4)、Rotary Table（32 tubes）（Optional）
17 Detection Speed Maximum 30000/s
18 Minimum Sample Volume 90μL
19 Measuring Range Maximum 107
20 Threshold Support double threshold，remove most of cell debris
21 Fluorescence compensation Support auto FL compensation，offline compensation， visualize compensation tool
22 Item template Support template ， Acquisition Condition/FL compensation with single-click
Cyto Analyze,Cell Analyzer,Flow Cytometry Analyzer,Dna Cell Analyzer,Analyzer For Cell,Flow Cytometer
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